A colored number corresponding to the type you are counting will be displayed on the image every time you click, and the corresponding counter is updated.Īdd the following macros to ImageJ/macros/StartupMacros.txt, restart ImageJ, and you will be able to change the counter type by pressing "1", "2", "3", etc. Select the type you want to count, and count by clicking on the feature in the image. Note that at any time you can add types or remove them. Click initialize, now you are ready to count features. Open the Cell Counter plugin and the image/stack you want to count (if the Cell Counter plugin is already open you don't need to open a new instance). Measure.: Measures the pixel value at each marker and displays a result window showing: Type - Slice - X coordinate - Y coordinate - Pixel Value.Export Image: Makes a copy of the counter image with the markers written on it (only the current slice is copied in case of a stack).Load Markers: Loads stored marker data from an XML file - Only available when running java 1.4 or higher.Save Markers: Exports the marker data to an XML file - Only available when running java 1.4 or higher.In case of a stack the counts per slice and the totals are displayed. Results: Shows the counter results in the ImageJ results table.When checked, the marker of the currently selected type closest to the mouse cursor will be deleted when you click. Delete mode: toggles between insert and delete mode.I usually tick Display Results (show every lable’s area) and Summarize (the sum area of the selected). For instance, in the sample image, if no selection is made, ImageJ will caculate all the red area. Set the minimum size and maximum pixel area size to exclude anything that is not an object of interest in the image. This will provide you with information about each particle in the image. Initialize: Initializes the current image or stack for counting, this will create a duplicate image/stack titled "counter window" - when keep original is checked, the source image remains open Select Rectangular tool in the tool bar to restrict the area you want to calculate. To analyze the particles in a segmented image, use the menu command Analyze Analyze particles.Opens off-screen on 1024 pixel wide and smaller monitors. May not work correctly after using Load Markers to load more than 8 counter types from an XML file.
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